ABSTRACT
OBJECTIVE@#To establish a primary culture method of human omental preadipocytes.@*METHODS@#Using enzyme-digesting method, fibroblast-like cells from the human omental adipose tissues were cultured, and then differentiated by conditional medium, and identified by oil red O staining.@*RESULTS@#The cultured cells were highly homogeneous, and highly proliferative in 4-5th generation. During the process of induction by conditional medium, the cells became round-like and larger, and more adipose droplets were aggregated. By oil red O staining, we confirmed the differentiated cells were mature adipocytes.@*CONCLUSION@#In human omental adipose tissues, there are some preadipocytes, which can differentiate into mature adipocytes with appropriate stimulus.
Subject(s)
Adult , Humans , Male , Adipocytes , Cell Biology , Cell Differentiation , Cells, Cultured , Culture Media, Conditioned , Omentum , Cell BiologyABSTRACT
OBJECTIVE@#To explore the effect of simvastatin on the cell cycle and caspase-3 expression in human omental preadipocytes.@*METHODS@#The preadipocytes were randomly divided into a blank group, a 10(-5) mol/L simvastatin group, and a 10(-4) mol/L simvastatin group. Each group was incubated with different concentrations of simvastatin for 48 hours. MTT method was used to analyze the effect of simvastatin on the proliferation. Distribution of the cell cycle was measured by flow cytometric. Caspase-3 expression was examined by cyto- immunochemistry.@*RESULTS@#After being induced to differentiate for 16 days the human omental preadipocytes developed to mature adipocyte penotypes with lipid droplet. Simvastatin 10(-4) mol/L had significant anti-proliferation effect. Flow cytometric analysis showed the cell cycle was blocked in G0/G1 phase and caspase-3 positive cells increased dramatically.@*CONCLUSION@#Simvastatin may block the cells in G0/G1 phase, and induce caspase-3 expression, which may trigger apoptosis.
Subject(s)
Humans , Adipocytes , Cell Biology , Anticholesteremic Agents , Pharmacology , Apoptosis , Caspase 3 , Caspases , Genetics , Cell Cycle , Cell Proliferation , Cell Separation , Cells, Cultured , Omentum , Cell Biology , Simvastatin , PharmacologyABSTRACT
Objective To observe the effects of genistein and 17?-estradiol on microstructure of cancellous bone in ovariectomized (OVX) rats.Methods Ninty 7-month-old SD rats were randomly divided into baseline group,ovariectomized (OVX),sham-operated (SHAM),17?-estradiol treated (10?g?kg~(-1).day~(-1),EST) and genistein treated (5 mg?kg~(-1)?day~(-1),GEN) groups,and were killed at the beginning of the experiment,the 3rd and 15th week after operation.MicroCT scanning was performed on the left tibia in vitro.The regions involving 0.5 mm slice thickness and 1.6 mm distal to the tibial growth plate were selected as the regions of interest.Results At the 3rd week after operation,the tissue bone mineral density (tBMD) and trabecular thickness (sTh.Th) in group GEN were significantly higher than those in OVX and EST groups (all P